Bioluminescence Assays and Reagents
Biotium Coelenterazine 400A (DEEPBLUEC, A TRADEM
Coelenterazine 400a, also called DeepBlueC™, is a coelenterazine derivative that serves as a substrate for Renilla luciferase (Rluc) and generates an emission peak centered around 400 nm. It is the preferred Rluc substrate for BRET studies because it has minimal interference with the emission of the GFP acceptor. Properties : Off-white to pinkish solid soluble in MeOH or EtOH; Store at -20°C, protect from light; C26H21N3O; MW: 391.46. DeepBlueC is a trademark of BioSignal Packard.
Biotium Firefly & Renilla Luciferase Single Tube Assay Kit, 50 Assays
The Firefly & Renilla Luciferase Single Tube Assay is a combined luciferase assay allowing sequential measurement of Firefly and Renilla luciferase activity in the same sample with high sensitivity and linearity. Firefly luciferase activity is measured first, then Renilla Luciferase Assay Buffer 2.0 is added to the sample to simultaneously quench firefly luciferase activity and measure Renilla luciferase activity. The Renilla Luciferase Assay Buffer 2.0 quenches the firefly luciferase activity to the level of untransfected cells, allowing sequential measurement of firefly and Renilla luciferase activity in the same sample. In addition to providing a simplified, single tube assay, the kit features Biotium’s Aquaphile™ coelenterazine, a water soluble substrate that can be stored at -20°C with minimal evaporation, unlike methanol solutions of coelenterazine This is a trial size kit with reagents sufficient for 50 assays for plate sizes ranging from 96-well to 24-well.
Biotium Firefly & Renilla Luciferase Single Tube Assay Kit, 1000 Assays
The Firefly & Renilla Luciferase Single Tube Assay is a combined luciferase assay allowing sequential measurement of Firefly and Renilla luciferase activity in the same sample with high sensitivity and linearity. Firefly luciferase activity is measured first, then Renilla Luciferase Assay Buffer 2.0 is added to the sample to simultaneously quench firefly luciferase activity and measure Renilla luciferase activity. The Renilla Luciferase Assay Buffer 2.0 quenches the firefly luciferase activity to the level of untransfected cells, allowing sequential measurement of firefly and Renilla luciferase activity in the same sample. In addition to providing a simplified, single tube assay, the kit features Biotium’s Aquaphile™ coelenterazine, a water soluble substrate that can be stored at -20°C with minimal evaporation, unlike methanol solutions of coelenterazine This kit has reagents sufficient for 1000 assays for plate sizes ranging from 96-well to 24-well.
Biotium Firefly & Renilla Luciferase Single Tube Assay Kit, 150 Assays
The Firefly & Renilla Luciferase Single Tube Assay is a combined luciferase assay allowing sequential measurement of Firefly and Renilla luciferase activity in the same sample with high sensitivity and linearity. Firefly luciferase activity is measured first, then Renilla Luciferase Assay Buffer 2.0 is added to the sample to simultaneously quench firefly luciferase activity and measure Renilla luciferase activity. The Renilla Luciferase Assay Buffer 2.0 quenches the firefly luciferase activity to the level of untransfected cells, allowing sequential measurement of firefly and Renilla luciferase activity in the same sample. In addition to providing a simplified, single tube assay, the kit features Biotium’s Aquaphile™ coelenterazine, a water soluble substrate that can be stored at -20°C with minimal evaporation, unlike methanol solutions of coelenterazine This kit has reagents sufficient for 150 assays for plate sizes ranging from 96-well to 24-well.
Biotium Firefly Luciferase Assay Kit 2.0, 150 Assays
Firefly luciferase is widely used as a reporter for studying gene regulation and function, and for pharmaceutical screening. It is a very sensitive genetic reporter due to the absence of endogenous luciferase activity in mammalian cells or tissues. The enzyme is a 62,000 Dalton protein, which is active as a monomer and does not require subsequent processing for its activity. Firefly luciferase catalyzes ATP-dependent D-luciferin oxidation to oxyluciferin, producing light emission centered at 560 nm. This firefly luciferase assay kit is designed for simple and efficient quantitation of firefly luciferase reporter enzyme activity from cultured cells with high sensitivity and linearity. The kit is suitable for plate sizes ranging from 96-well to 24-well and has reagents sufficient for 150 assays.
Apexbio Technology LLC EZ Cap™ Cy5 EGFP mRNA (5-moUTP), 100ug (1mg/mL)
EZ Cap™ Cy5 EGFP mRNA (5-moUTP) will express enhanced green fluorescent protein(EGFP) once entering cells originally isolated from the jellyfish(Aequorea victoria). You can observe the green fluorescence at 509 nm. EGFP is usually used as a reporter for gene regulation and function study. It is applicable in assays for mRNA delivery, translation efficiency, cell viability and in vivo imaging etc. Other sizes are also available. Please inqury us for quote.
Apexbio Technology LLC EZ Cap™ Firefly Luciferase mRNA (5-moUTP), 5x1mg (1mg/mL)
EZ Cap™ Firefly Luciferase mRNA (5-moUTP) will express luciferase protein once entering cells, which is initially extracted from firefly Photinus pyralis. This enzyme catalyzes ATP-dependent D-luciferin oxidation and lead to yield chemiluminescence at about 560 nm wavelength. Firefly Luciferase is a frequently used bioluminescent reporter for gene regulation and function study. It is applicable in assays for mRNA delivery, translation efficiency, cell viability and in vivo imaging etc. EZ Cap™ Firefly Luciferase mRNA (5-moUTP) is provided at a concentration of 1mg/ml. It is co-transcriptional capped by EZ Cap™ Reagent AG (3' OMe) (Catalog No.B8178) which generates a cap 1 structure with high efficiency. Other sizes are also available. Please inqury us for quote.
Apexbio Technology LLC Firefly Luciferase mRNA (ARCA, 5-moUTP), 100ug (1mg/mL)
Firefly Luciferase mRNA (ARCA, 5-moUTP) expresses luciferase protein which is initially extracted from firefly Photinus pyralis. The enzyme catalyzes ATP-dependent D-luciferin oxidation to yield oxyluciferin, a singlet-excited compound that emits light when returning to its ground state. Firefly Luciferase is a frequently used bioluminescent reporter for gene regulation and function study. It is applicable in assays for gene expression, cell viability and in vivo imaging etc. Our Firefly Luciferase mRNA is provided at a concentration of 1mg/ml, it is ARCA capped, polyA-tailed and incorporated with modified nucleotides. The capping of ARCA (anti-reverse cap analog) ensures high translation efficacy. Poly (A) tail plays an important role in enhancing the efficiency of translation initiation. Other sizes are also available. Please inqury us for quote.
Invivogen J774-Dual Cells, 3-7 x 10e6 cells
J774-Dual cells have been derived from the mouse J774.1 macrophage-like cell line by stable integration of two inducible reporter constructs.
Invivogen HCT116-Dual Cells, 3-7 x 10e6 cells
HCT116-Dual cells are adherent epithelial cells that have been derived from the human HCT116 colorectal carcinoma cell line by stable integration of two inducible reporter constructs. HCT116-Dual™ cells express numerous pattern recognition receptors (PRRs), including the NOD-like receptors (NLRs) NOD1 and NOD2, the RIG-I-like receptor (RLR) RIG-I, and the Toll-like receptors (TLRs) TLR3 and TLR5 but not TLR2 or TLR4.
Gold Biotechnology Inc D-Luciferin Firefly, free acid (Proven and Published™) 1 g
Luciferin is a common bioluminescent reporter used for in vivo imaging of the expression of luciferase. This substrate for the firefly luciferase enzyme utilizes ATP and Mg2+ as cofactors to emit a characteristic yellow-green emission in the presence of oxygen, which shifts to red light in vivo at 37°C. Through the utilization of ATP, the reaction can be further used to indicate the presence of energy or life in order to function as a life-death stain.