Bioluminescence Assays and Reagents
Cayman Chemical CyprIdIna LucIfRIntrIfluor 1mg
A chemiluminescent substrate of luciferase; upon hydrolysis by luciferase, em = 465 nm; has been used in ERα reporter assays for the discovery of ERα-activating compounds in vitro and in BRET assays for tumor detection in vivo
Molecular Devices Reporter Assay Kit, Dual Luciferase, Flash, 50 Reactions
The SpectraMax DuoLuc Reporter Assay Kit is an evaluation kit for sensitive, sequential measurement of firefly and Renilla luciferase activity in mammalian cells. The kit uses flash-type luminescence and is optimized for microplate readers with injector capability, providing reagents for cell lysis and substrate reactions for approximately 50 assays.
- Enables sequential measurement of firefly and Renilla luciferase using flash-type luminescence.
- Provides reagents for approximately 50 reactions.
- Optimized for use on microplate readers with injector capability.
- Typical workflow uses about 20 μL of cell lysate per well and 100 μL injection volumes for each substrate.
- Includes reagents for cell lysis and substrate preparation to support rapid assay setup.
BIOHIPPO EF1a-KRAS-G12D-V5/GFP
EF1a-KRAS-G12D-V5/GFP lentiviral vector expressing V5 under EF1a promoter Each vial provides a nominal titer of 2x10E6 transducing units TU Human KRAS G12D ORF cDNA lentivirus Lentiviral particles packaged with expression vector for G12D mutant of human KRAS proto-oncogene GTPase (KRAS) transcript variant a An EF1 promoter drives expression of KRAS G12D fused to a C-terminal V5 tag and a GFP reporter which is separated from the ORF cDNA by a self-cleaving peptide These lentiviral particles allow for easy transduction and high stable expression of the indicated ORF cDNA in mammalian cells The lentiviral vectors have been optimized to provide high gene expression and reliable genome integration allowing for easy establishment of long-term stable cell lines with consistent read out of fluorescent (GFP RFP) or luminescent (firefly luc) reporters
Vector Biolabs AP1-Luc Adenovirus (Ad-AP1-Luc)
Expression of the Photinus pyralis (firefly) luciferase gene is controlled by a synthetic promoter that contains direct repeats of the transcription recognition sequences for the activator protein 1 (AP1).When this Luciferase reporter adenovirus and an uncharacterized gene are cotransfected into mammalian cells, the activation of endogenous protein kinases initiated by the cotransfected gene product will result in the activation of corresponding trans-activators which in turn stimulate luciferasae expression.
BIOHIPPO EF1a-KRAS-V5/GFP
EF1a-KRAS-V5/GFP lentiviral vector expressing V5 under EF1a promoter Each vial provides a nominal titer of 2x10E6 transducing units TU Human KRAS ORF cDNA lentivirus (wild type) Lentiviral particles packaged with expression vector for wild type human KRAS proto-oncogene GTPase (KRAS) transcript variant a An EF1 promoter drives expression of KRAS fused to a C-terminal V5 tag and a GFP reporter which is separated from the ORF cDNA by a self-cleaving peptide These lentiviral particles allow for easy transduction and high stable expression of the indicated ORF cDNA in mammalian cells The lentiviral vectors have been optimized to provide high gene expression and reliable genome integration allowing for easy establishment of long-term stable cell lines with consistent read out of fluorescent (GFP RFP) or luminescent (firefly luc) reporters
ABclonal Technology PEF1 Rabbit mAb
This gene encodes a calcium-binding protein belonging to the penta-EF-hand protein family The encoded protein has been shown to form a heterodimer with the programmed cell death 6 gene product and may modulate its function in Ca(2 ) signaling Alternative splicing results in multiple transcript variants and a pseudogene has been identified on chromosome 1